Photographic techniques for recording chromosome banding patterns.

Application of chromosome banding techniques to the study of primary chromosome structural changes.

Some comments are provided for the benefit of new workers on the use of chromosome banding techniques for the recognition, classification, scoring, and break point location of primary chromosomal structural changes.

Karyotype Analysis and Chromosome Banding

Nearly all methods of chromosome banding rely on harvesting chromosomes in mitosis. This is usually achieved by treating cells with tubulin inhibitors, such as colchicine or demecolcine (colcemid), that depolymerize the mitotic spindle and so arrest the cell at this stage. Excessively long incubations with Colcemid result in overcondensed chromosomes that band poorly and moreover some cell type...

Photographic Techniques in Experimental Hydraulics

Fluorescence and Giemsa Banding Techniques

The C group chromosomes in man cannot be distinguished solely on morphological grounds, thus, the nature of an extra C chromosome remains unknown unless it is an X chromosome, which can be identified by its late DNA-replication pattern. Extra autosomes belonging to the C group have been observed with or without mosaicism in a wide variety of blood disorders (cf, de la Chapelle et al, 1970; Hell...

A new chromosome banding technique, spectral color banding (SCAN), for full characterization of chromosomal abnormalities.

We have developed spectral color banding (SCAN) as a new chromosome banding technique based on spectral analysis of differentially labeled chromosome band-specific painting probes. In this study, we succeeded in displaying a multicolor banding pattern for chromosome 3, which was almost identical to the pattern obtained with the corresponding G-banding. We applied this method to metaphase cells ...